Consulta: creatorFacets:"Razmkhah,Farnaz"
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Data/hora: 19/05/2024 01:26:11

MicroRNA-21 over expression in umbilical cord blood hematopoietic stem progenitor cells by leukemia microvesicles
Provedor de dados: Genetics and Molecular Biology
Autores: Razmkhah,Farnaz; Soleimani,Masoud; Ghasemi,Sorayya; Kafi-abad,Sedigheh Amini.
Abstract Microvesicles are able to induce the cell of origin’s phenotype in a target cell. MicroRNA-21, as an oncomir, is up-regulated in almost all cancer types such as leukemia which results in cell proliferation. In this study, we examine the ability of leukemia microvesicles to induce proliferation in hematopoietic stem progenitor cells (HSPCs) via microRNA-21 dysregulation. Herein, leukemia microvesicles were isolated from HL-60 and NB-4 cell lines by ultracentrifugation, and then their protein content was measured. Normal HSPCs were isolated from umbilical cord blood samples by a CD-34 antibody. These cells were treated with 20 and 40 μg/mL leukemia microvesicles for 5 and 10 days, respectively. Cell count, CD-34 analysis, and a microRNA-21 gene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leukemia; Microvesicles; Hematopoietic stem cells; MicroRNA-21.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300465